Invertase Enzyme, also called beta-fructofuranosidase which can be isolated from S. cerevisiae and other microorganisms. Hydrolysis from sucrose to fructose and glucose is triggered by this very enzyme. The production of inverted sugar is one of the invertase’s numerous applications. β-Fructofuranosidases are certain enzymes that are capable of hydrolyzing substrates with terminal fructose. Most β-fructofuranosidases have been shown to hydrolyze sucrose to release glucose and fructose and to obtain fructosyltransferase activity for the synthesis of short-chain fructooligosaccharides. Due to its sweetening effects, which are known to be more than that of sucrose, it carries great industrial significance and has high prospects for its use in biotechnology.